Kisspeptin-10 Reconstitution Calculator

No. Kisspeptin-10 has no FDA or EMA approval for any indication; human use remains confined to clinical trials and laboratory research. The only marketed drug acting on adjacent kisspeptin/NKB circuitry is fezolinetant (Veozah), an NK3R antagonist approved for menopausal vasomotor symptoms. Kisspeptin-10 is on the WADA 2024 Prohibited List and is detectable in urine. Compounded or gray-market use is off-label and unregulated (practitioner consensus).

KP-10 is the C-terminal decapeptide shared by all kisspeptin isoforms and fully activates the kisspeptin receptor KISS1R. It drives GnRH release, raising LH, FSH, and testosterone (human RCT). In healthy men, 8-h subcutaneous infusions of 1.25–10 nmol/kg/h increased LH, FSH, and testosterone dose-dependently (P<0.0001 vs placebo); daily 8-h infusions at 150 nmol/h (~195 μg/h) sustained LH elevation for 12 days (day 1: +1.68, day 12: +1.14 IU/L). A 1 nmol/kg bolus (~1.3 μg/kg) remained effective after 12 days. Continuous IV 12.5 μg/kg/h raised LH 5–8× by 12–20 h, then declined 13–47% through tachyphylaxis. IV 1.5 μg/kg/h for 12 h reactivated LH/FSH/estradiol in two women with hyperprolactinemic amenorrhea. Community bolus dosing: 100–250 μg subcutaneously 1–3×/day (community protocol).

SC is the practical standard (community protocol). IV suits clinical/provocative use; intranasal has only rat pharmacology; oral is non-viable.

No. Kisspeptin-10 is a physiological inhibitor of primary human trophoblast invasion (in-vitro). No human pregnancy safety data exist, and placental kisspeptin signaling shifts dramatically across gestation. Treat as contraindicated and discontinue when attempting conception (practitioner consensus).

Pulsatile exposure avoids desensitization. Eight-hours-on/16-hours-off SC dosing sustained gonadotropin responses for 12 days, with the receptor still functional at day 12. Continuous 5-day infusion blunted gonadotropin responses while testosterone remained elevated; 24-h IV infusion produced 13–47% tachyphylaxis. Chronic high-dose intermittent dosing in prepubertal rats caused dose-dependent Leydig cell degeneration and reduced testosterone. Community practice: cycle 4–12 weeks using intermittent pulses, then off-periods (community protocol).

Kisspeptin-54: half-life ~32 min vs ~4 min for KP-10 in mice; crosses the blood–brain barrier and sustains LH release far longer after one injection. In an RCT (n=62) in women at high OHSS risk, two SC doses of KP-54 (9.6 nmol/kg, 10 h apart) triggered oocyte maturation: oocyte yield ≥60% in 71% vs 45% of patients, with 0% moderate/severe OHSS in the double-dose arm. GnRH (gonadorelin): acts directly on the pituitary; produced a 3.5× higher LH peak than KP-10 and induced ovulation in luteal-phase cattle where KP-10 did not. Kisspeptin is more physiological, acting through endogenous GnRH stores. Kisspeptin-13: most favorable metabolic profile among isoforms: β-cell proliferation and no glucose intolerance in mice, whereas KP-10 and KP-14 impaired glucose tolerance.

Acute tolerability is favorable: a 14-day IV dog study found a NOAEL of 1,000 μg/kg/day with no organ toxicity, and human studies report no serious adverse events at LH-stimulating doses. Preclinical flags for repeated long-term use: KP-10 is vasoconstrictive and pro-atherogenic (increased monocyte adhesion, foam-cell formation, and aortic plaque progression/instability in ApoE−/− mice, reversed by antagonist P234 (animal) ); it increased myocardial collagen via FAK signaling in human cardiac fibroblasts (in-vitro). Non-pulsatile stimulation desensitizes the axis, and chronic exposure damaged Leydig cells in prepubertal rats.

No formal reconstituted-stability data exist. KP-10 undergoes rapid N-terminal proteolysis in plasma (in-vitro), and a seized commercial liquid vial showed tryptophan oxidation. Store lyophilized powder desiccated at −20°C; after reconstitution with bacteriostatic or sterile water, refrigerate at 2–8°C and discard after ~30 days; keep liquid cold in transit (community protocol). Expect unrefrigerated liquid to degrade within hours-to-days.